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The animal was then given an anaesthetic overdose (pentobarbital sodium, 200 mg/kg, i.v.), the heart was excised, briefly frozen and sectioned into 56 transverse slices from apex to base

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There was no significant difference in plasma total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol, leptin, adiponectin, resistin or interleukin-6 in Gipr Syn/ mice compared to Gipr fl/fl mice, although plasma tumour necrosis factor (TNF) and monocyte chemoattractant protein-1 (MCP-1) were lower in Gipr Syn/ mice than in Gipr fl/fl mice (Extended Data Fig