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Subsequently, cells were washed with PBS buffer and incubated with MTT reagent (5 mg/mL in PBS, pH 7.4) for 4 h

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Obes Surg 23(11):19221933 Majid SF, Davis MJ, Ajmal S et al (2022) Current state of the definition and terminology related to weight recurrence after metabolic surgery: review by the POWER Task Force of the American Society for Metabolic and Bariatric Surgery

In patients with PCOS, estrogen and androgen metabolism are abnormal