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This can be achieved by increasing the salt concentration or incorporating mild detergents in the wash buffer

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In a manner similar to that observed in liver, the accumulation of intramyocellular DAG impairs insulin signaling and muscle glucose uptake by activating PKC (muscle-type nPKC) [112,113], which elicits the phosphorylation of IRS-1 at Ser1101 and blocks the insulin-stimulated phosphorylation of IRS-1 [114,115]

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Perovic, D., et al

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doi: 10.3164/jcbn.1106SR 104 MollFWalterMRezendeFHelfingerVVasconezEDe OliveiraTet al

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