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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)

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Each mouse was recorded for at least 30 minutes, with the average amplitude and frequency calculated automatically using LabChart 8 software (AD Instruments)

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While some ingredients used in IV hydration may be commonly used in clinical settings, your provider can help determine what is appropriate for your individual needs