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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

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Such models will further contribute to the investigation of genetic and environmental factors in the autoimmune development of thyroid disorders and, as such, will be an important tool for therapeutic development (Capuzzo 2021)

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