A protein aliphatic index is described as the relative volume occupied by aliphatic side chains (leucine, isoleucine, valine, and alanine) and is viewed as a favorable factor for the rise of thermostability in globular proteins
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2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)
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