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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)

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189 HSP90 also promotes the activation of oncogenic protein kinases such as JAK2/STAT3, PI3K/AKT, and MAPK, facilitating cancer progression

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