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Sarcocystis neurona grows relatively slowly in host cells and the merozoites are also released from the cells rather slowly

By inhibiting overactivity within the axis, it reduces levels of key hormones such as corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), CORT, and orexin-A

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Nonetheless, its systemic injection in mice has demonstrated significant adverse effects, including renal failure and hemolysis, presenting a hurdle for in vivo application

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Blood samples were fractionated by centrifugation at 1500 g at room temperature for 10 min