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supernatants were quantified by BCA assay (Thermo, 23227) and denatured at 95C for 5 min in 5 SDS loading buffer containing dithiothreitol (DTT, 100 mM)

Creatinine, DCD, and DHT are by-products considered impurities found in creatine as a result of manufacturing processes

These alterations not only extend its duration of action but also enable IGF-1 LR3 to circumvent binding with IGF-binding proteins, which typically impede the biological availability and efficacy of IGF-1

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