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& Mootha, V
J Cell Physiol (2011) 226:265165
Having large meals will stretch your stomach and make the discomfort even worse
After washed twice with cold PBS, the cells were suspended with 200 l of PBS, and supplemented with 10 l of RNase A stock (#10405ES03, Yeasen) solution and incubated 60 min at room temperature
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It should also be emphasised that currently we do not recommend the use of simvastatin (particularly in a dose of 80 mg due to adverse reactions), but if the treatment goal has been achieved and the patient tolerates it well, there is no need to change it
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