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The physical disruption denatures the protein irreversibly

For all fermentor-based experiments a CEN.PK113-1A strain background was used

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Violent mechanical agitation should be completely avoided

6a, b), as we observed in previous experiments with 3-PLG-A-GSH [24], and with serum albumin cargo loaded A-GSH targeted vesicular nanoparticles [32, 33]

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