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Blood from the inferior vena cava was collected and kept in EDTA-coated blood tubes (K 2 EDTA Capillary Collection Tube, MiniCollect ) for immediate haematology analysis and for the estimation of biochemical parameters

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Groups were treated with VEH, GIPR-antag, LIRA and LIRA + GIPR-antag

To study chronic long-term effects, CNO was administered i.p

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Understanding the Gut-Brain Connection Johns Hopkins Medicine Synergistic Combinations for Tier 3 The Metabolic Restoration Stack: Retatrutide or Tirzepatide (comprehensive metabolic improvement) Tesamorelin (visceral fat reduction) SLU-PP-332 or BAM15 (metabolic enhancement and energy restoration) BPC-157 (gut-metabolic axis restoration) Thymosin Alpha-1 (immune-metabolic optimization) This aggressive stack addresses the multiple dysfunctions present in metabolic disease: insulin resistance, chronic inflammation, mitochondrial dysfunction causing low energy, and gut dysbiosis contributing to brain fog