Once the optical density (OD 600 ) reached approximately 0.6, the culture was induced with 1 mM IPTG (Isopropyl -D-1-thiogalactopyranoside) and incubated for an additional 23 h at 37 C in a shaker
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While Hydroxocobalamin offers longer retention in the body, Methylcobalamins enhanced bioavailability ensures quicker absorption and utilization
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