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Cultures were then diluted 1:500 to 1:1,000 in TSB or Seahorse XF DMEM (Agilent) supplemented with 100 mM glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, and dispensed into poly-D-lysine (PDL)-coated Seahorse XF HS miniplates (100 l per well)
ROS initiate autophagy by activating the AMPK pathway and inhibiting the mTOR signal
Drugs R D 6:5355
Autophagic cell death
Protocols are personalized based on goals, baseline lifestyle load, skin concerns and response
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