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There are other mechanisms that provide the protective effect of SOD as an antioxidant enzyme

Wound-healing assay NHEK (4 10 5 cells) were plated into 96-well plates and incubated in KBM2 supplemented with 0.4% bovine pituitary extract, 0.125 ng ml 1 rh EGF, 5 g ml 1 rh insulin, 0.33 g ml 1 hydrocortisone, 0.39 g ml 1 epinephrine, 10 g ml 1 transferrin and 0.06 mM CaCl 2 for 24 h, to allow the formation of a confluent monolayer

The analytical standards in FDA guidance on testing methods are the benchmark for this kind of work

Previous studies reported the antioxidant properties of Epitalon by increasing SOD activity and reducing the levels of lipid peroxidation (LPO) products, diene conjugates, and ROS [49,50,51]