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Water content was calculated according to the following formula: [(wet weight dry weight)/(wet weight) 100 (%)] ( Evaluation of Neurological Deficits Neurological deficits were evaluated using a five-point scale established by Immunohistochemistry and Nissl Staining Immunohistochemistry experiments were performed using the UltraSensitiveTM SP (Mouse/Rabbit) immunohistochemistry Kit (Mixim, Fu Zhou, China), following the manufacturers instructions
doi: 10.3390/microorganisms12112159 35 KopczyskaJKowalczykM
The fluorescence was then analyzed using flow cytometer and CLSM
Results were additionally confirmed using glioblastoma neurospheres in coculture and engrafted in mice
The ROS-responsive size changes of Mn/TI@mHSA NPs were assessed by monitoring their size via DLS and TEM following incubation in 1 or 10 mM H 2 O 2 for 12 h
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