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The lentiviral particles for CBS OPT and L2HGDH OPT constructs were generated similarly with the packaging system as those for sgRNA but selected with puromycin at a final concentration of 1.5 g/ml

Time Your Nutrition Around Workouts Since appetite is lower, be intentional: Eat protein before or after training Include some carbs around workouts for energy Dont train completely fasted if performance is suffering 5

Consult with your physician before starting any medical therapies

Dehydration: Alcohol makes the body lose more fluids, which can make you feel more lightheaded or dizzy