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The lack of a well-defined primary receptor for DSIP has hampered mechanistic research if such a receptor exists, it has not been convincingly identified and cloned, leading some researchers to propose that DSIP may work through non-receptor-mediated mechanisms or through promiscuous interactions with multiple receptor systems

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A May 2025 study in Molecular Therapy from the INc-UAB showed that gene therapy increasing secreted klotho (s-KL) in mice extended lifespan by 15-20% while improving muscle, bone, and cognitive health at 24 months of age (equivalent to ~70 human years)

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Each time a dose is withdrawn with a sterile needle, the benzyl alcohol prevents microbial contamination from multiplying in the vial