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Segurana de Alimentos: Foca especificamente nas prticas e regulamentaes adotadas desde a produo agrcola at o consumo final

Day 10 cultured SCD erythroblasts were treated with 75 M hemin (#51280, Sigma) (dissolved in 0.25 M NaOH then neutralized with HCL to pH7.4), 50 M dimethyl fumarate (#242926, Sigma) (dissolved in DMSO), 0.5 mM (2S)-Tetrahydro-5-oxo-2-furancarboxylic acid (TFMB-L2HG) (#SML2563, Sigma) (dissolved in DMSO), 0.5 mM S-(5-Adenosyl)-L-methionine iodide (SAM) (#A4377, Sigma) (dissolved in water), and cultured under hypoxia (1% O 2 ) using Heracell 150i incubator equipped with an Oxygen (O 2 ) sensor and Nitrogen (N2) gas supply and analyzed on day 12 of culture

Funding This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC, Discovery grant nos

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Photos were taken and lungs were solubilized in 1 % Triton x-100, 20 mM Tris, 0.15 M NaCI, 2 mM EDTA, and 0.02% sodium azide