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In vitro experiments demonstrated that Akt inhibition markedly upregulated the expression of FTL, FTH, TFR1, and Poly (rC)-binding protein 1 (PCBP1), downregulated FPN1 expression, increased APP expression, enhanced iron storage and uptake, and decreased iron efflux, thereby aggravating neurological injury in SH-SY5Y and C2C12 cells ( 5.2 Exercise regulates oxidative stress As a principal driver of ferroptosis, oxidative stress is defined as a physiological or pathological condition wherein the production of ROS and reactive nitrogen species (RNS) under endogenous or exogenous stimuli surpasses the neutralising capacity of the antioxidant defence system, thereby causing oxidative damage (Zhang et al., 2024)

Overall, this study supports the efficacy and favourable effects of NAC supplementation on improvement of reproductive system function

Phase 2: maintenance (ongoing) After the loading phase, transition to maintenance dosing

( E ) Densitometry results showing increases in intracellular periostin (POSTN), CCL26, and iNOS following treatment with erastin ( n = 34)

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