Provider Assessment and Shared Decision-Making for GLP-1 Treatment Safety Treatment initiation decisions should be collaborative and based on individual risk-benefit analysis
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doi:10.1016/j.obpill.2025.100181 Fitch A, Gigliotti L, Bays HE
Cells Vero African green monkey kidney cells were cultured at 37C in a 5% CO2 atmosphere in Eagles Minimum Essential Medium (MEM, HyClone) containing 1.2 mg/ml NaHCO3 and supplemented with 6% (v/v) foetal bovine serum, 2 mM glutamine, 100 IU/ml penicillin and 100 g/ml streptomycin
Baseline measurements were taken after an initial 5 min incubation with coelenterazine-h or NanoGlo-containing HBSS, after which cells were treated with either a vehicle (PBS) or the respective ligands
