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All infections were performed using a standardized inoculum of 1 10 7 colony-forming units (CFU) suspended in phosphate-buffered saline (PBS) and administered via the intranasal route using 50 l/nostril

[DOI] [PubMed] [Google Scholar] 89.Bailey L.B., Stover P.J., McNulty H., Fenech M.F., Gregory J.F., III, Mills J.L., Pfeiffer C.M., Fazili Z., Zhang M., Ueland P.M., et al

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A three-step amplification protocol protocol was employed, with glyceraldehyde-3-phosphate dehydrogenase ( GAPDH) as the internal control gene

Khaliq S, Haider S, Naqvi F, Perveen T, Saleem S, Haleem DJ

If irritation or an allergic reaction occurs, use of the product should be discontinued immediately, and medical advice should be sought