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Enzyme activities in the supernatant were analyzed with a UV spectrophotometer and calculated per gram of intestinal contents or mucosa in unit U/g (Wu et al., 2021

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doi: 10.3390/ijms26115173

Furthermore, the induction of CYP2E1 during chronic alcohol consumption generates massive amounts of ROS, which likely accelerates mitochondrial dysfunction and ER stress, and inflammatory signaling