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Here, we benchmarked several engineered laccase templates 22,23,24,25 , identified an ancestral fungal variant with slight activity in HEK293T cells and performed extensive directed evolution on the yeast surface to improve its activity (Fig

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As such, a single residue at the C-terminus of the S tail is a critical determinant of SARS-CoV-2 propagation and infectivity and explains why acidic C-terminal residues have not been reported in CoV S molecules that display a dibasic motif