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It is produced in three main locations: enteroendocrine L-cells of the intestinal mucosa, pancreatic -cells, and certain neurons within the nucleus of the solitary tract in the brainstem

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Sample size Sample size was calculated by using Power Analysis and Sample Size (PASS) Software (version 15, 2017)

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Prebiotics are substances selectively utilized by host microorganisms to promote health benefits, including the modulation of gut microbiota and the production of beneficial metabolites such as short-chain fatty acids (SCFAs) and tryptophan (Galica et al., 2022)