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With a genetic disease like autism, theres the concept of a double hit. You might have a gene mutation that gets you close to the threshold for autism, and then you experience another insult (medical event or environmental exposure) that confounds it and pushes you over the line

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In vivo and point-of-care (POC) approaches Bedside or minimally invasive POC strategies (electrochemical cartridges/biosensors and selected breath or surface readouts) can track trajectory and therapy response but rarely resolve species or compartments