Cytochemical SA--galactosidase assay Cells were seeded in six-well plates, fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min, washed with 1 mM MgCl 2 /PBS (pH 6.0) and then incubated with X-Gal staining solution (1 mg ml 1 of X-Gal (Thermo Scientific, 2737087), 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 ) for 68 h at 37 C
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In one study, obese Zucker rats received daily oral doses of AOD 9604 for 19 days
These criteria vary widely between employers, state Medicaid programs, and individual plans
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Tissue distribution data suggest preferential accumulation in adipose depots, consistent with the mechanism-based selectivity investigators have come to expect