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Samples were denatured by boiling at 95 C for 10 minutes, loaded onto a 4% to 12% gradient gel (Thermo Fisher Scientific), and separated by electrophoresis

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3 Cardiovascular effects of GLP-1 receptor agonists: clinical evidence beyond glycemic control Since the FDA mandate in 2008 to demonstrate cardiovascular safety for new glucose-lowering agents, a series of large randomized cardiovascular outcome trials have evaluated GLP-1 RAs in patients with T2DM, many of whom had established atherosclerotic cardiovascular disease (ASCVD) or multiple risk factors

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The clinical evidence consistently shows that tirzepatide produces greater weight loss than semaglutide, and for patients who need to lose 20% or more of their body weight to reach a healthy range, that additional efficacy can be clinically meaningful