(16)Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (16) Preparation of Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (15) In step (15) of Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (20DMF)520DMF33DMF221 Add capping reagent (20% piperidine in DMF solution), react at room temperature for 5 minutes, drain, re-add capping agent at room temperature and react for 20 minutes, wash 3 times with DMF, wash 3 times with isopropanol, re-evaporated DMF Wash 2 times, wash 2 times with methanol, soak in methanol for 1 hour, drain, and dry the resin with nitrogen to form dry particles
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