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Measures precursor ion mass with high accuracy Confirms molecular weight of GLP-1 peptide Detects mass shifts due to modifications Key Insight: High-resolution MS enables differentiation of closely related peptide species

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Antigen retrieval was performed using sodium citrate buffer (0.1 M, pH 6, with 0.05% Tween-20) in a microwave, then washed with de-ionized water and PBS on a shaker

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