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The other consistent context in the preclinical literature is a metabolic environment the mouse studies pair the compound with diet/energy-balance conditions rather than testing it in isolation

Quantitation was performed using a 10-point internal calibration standard (ranging from 0.05 to 450 ng/mL) based on the primary MRM ion abundance of BPA to that of the associated IS (215.5 m/z)

Acknowledgements We acknowledge the National Centre for Aquatic Animal Health (NCAAH) for providing all the necessary facilities