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In vitro incubations were prepared with Supersomes (CES1b, CES1c and CES2), microsomes or S9 fractions (human, rat and minipig) in 0.1 M phosphate buffer pH 7.4 at 1 mg/ml protein concentration to encourage turnover of the amide aiding the detection of aromatic amine metabolites and confirmation of hydrolysis

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If your TSH rises, your levothyroxine dose may need adjustment downward

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