The membrane was treated with blocking buffer containing 3% skimmed milk, 350 mM NaCl, 10 mM Tris-HCl (pH 8.0) and 0.05% Tween-20 for 1 h and incubated with the indicated primary antibody overnight at 4C
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All descriptions of cellular signaling mechanisms, cytoprotection-associated endpoints, angiogenesis-associated findings, and tissue-level research observations are derived from published in vitro cell-based studies and selected preclinical experimental model research
In humans, diet is the primary route of non-occupational Pt exposure, with an average dietary Pt intake of 1.44 g per day estimated for adults in Sydney, Australia (Vaughan and Florence 1992)
Citrin/mitochondrial glycerol-3-phosphate dehydrogenase double knock-out mice recapitulate features of human citrin deficiency
Additionally, findings from 2 more studies have been reported in other platforms