These findings provide a clear foundation for clinical decision-making in severe obesity management
Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100
Animal study The C57BL/6 mice (male, 56 weeks old) were purchased from SPF (Beijing) Biotechnology Co., Ltd
The article says to contact a provider if leg cramps are severe, persistent, or accompanied by swelling, weakness, or dark urine, which it flags as possible signs of rhabdomyolysis or serious muscle injury
Yes, if sun protection isnt maintained
Preclinical studies in mice demonstrate significant reductions in fat mass and body weight without changes in food intake