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DIA MS2 scans were acquired in the Astral mass analyser with a 3 ms maximum injection time using a variable windowing scheme, using 2 Da windows from 380 to 680 m/z , 4 Da windows from 680 to 800 m/z and 8 Da windows from 800 to 980 m/z

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melanogaster that could conjugate 4-hydroxynonenal (4-HNE), an electrophilic end-product of lipid peroxidation [129]

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