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Lyophilization is the process of removing water from the preparation under reduced pressure, which ensures long-term stability of the substance

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Adequate B12 status helps maintain energy levels even during caloric restriction

Permeabilization was then performed using PBS containing 0.5% Triton X-100 (108,603, Merck, Darmstadt, Germany) for 1 h at room temperature, followed by washing twice for 5 min in PBS/BSA

Additionally, a 9-year-old girl showed a 95% reduction in seizure frequency after the use of the KD [153]

For instance, moderate acoustic trauma may lead to detachment or degeneration of high-threshold cochlear fibers from their corresponding inner hair cells