However, correctly determining the shelf life of a product can be a challenging endeavor, often due to a lack of resources and clear guidelines for conducting shelf life testing
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In 1891, it was found to be useful to treat malaria, thus making methylene blue "the first synthetic compound ever used as an antiseptic in clinical therapy and the first antiseptic dye to be used therapeutically," according to the authors of a Medicinal Research Reviews paper on the compound