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Briefly, for nicotinamide adenine dinucleotide (NAD + ) and nicotinamide adenine dinucleotide phosphate (NADP + ) determinations, 100 mg of frozen floret tissue was combined with 1 mL of 0.2 N HCl, vortexed for 5 min and centrifuged at 13,000 g and 4C for 10 min

For use only by healthcare professionals trained in injection techniques

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Sections were washed 6 10 min with KPBS and placed in goat anti-rabbit AF Plus 488 secondary antibody (1:500, Invitrogen) for 2 h at room temperature

Gastrointestinal changes like constipation and reduced appetite often develop alongside nausea during this early phase