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RA may also cause systemic multisystem injury and extra-articular manifestations

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Co-immunoprecipitation In brief, the extracts of SW620 cells were blocked with immunoglobulin G (IgG) or protein A/G agarose (BioWorld, Visalia, USA) for 2 h at 4C to remove nonspecific protein binding, and then incubated with anti-FBX8 (Abcam, Cambridge, UK) or anti-GSTP1 overnight at 4C