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Look for: Texture changes: Thickened, rubbery, or spongy areas indicate early lipohypertrophy

Meaning when you apply it, the copper isnt free, and wont be able to inhibit 5AR1

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For the TG clearance tests, mice were fasted overnight (~15 h), then administered 20% intralipid (Sigma-Aldrich) via oral gavage (15 l g 1 body weight) at ZT2