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Permeabilization and blocking were performed for 1 hour with PBS (with 0.4% (v/v) Triton X-100%) and 2% immunoglobulin G-free BSA (Jackson ImmunoResearch, USA)

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Similar to the ATP-binding site of hGSH synthetases, the adenosine in the Cp1B structure is sandwiched between the strands of two antiparallel -sheets (Fig