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Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)
Gipp et al., 1995)
The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH
Cells were stained with 0.5% of crystal violet in 20% of methanol aqueous solution for 10 min at room temperature
Prescription concentrations start at 1% and can go significantly higher
Tomosugi N, Yamamoto S, Takeuchi M, Yonekura H, Ishigaki Y, Numata N
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