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After three washes with 200 l prewarmed PBS, 100 l of 300 M prewarmed Hanks balanced salt solution (Gibco) was added, and luminescence was immediately measured at 37 C using a Synergy H1 reader

Documentation should include both objective measurements and subjective observations

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Each GLOW compound is reconstituted separately

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However, when too much everyday stress, lack of sleep, and unhealthy eating all come together, this protective shield becomes overloaded and the entire system is thrown out of balance