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The membrane was washed three times in Tris-buffered saline with Tween 20 (TBS-T) for 10 min, and treated with HRP-conjugated anti-rabbit or anti-mouse IgG (GE Healthcare) for 1 h

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both are tools that may help individuals achieve their health goals, but neither can necessarily solve their respective health conditions for everyone (Srinivasan et al., 2025)