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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
Here's how to verify: Check the testing lab Reputable labs include Janoshik Analytical, MZ Biolabs, and Colmaric Analyticals
AMPK activity is also allosterically regulated by AMP binding to the subunit (Figure 3)
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Cells were labelled with SNAP-Surface Alexa Fluor 546 (New England Biolabs) and SNAP-expressing fluorescent cells sorted by fluorescence-activated cell sorting (FACS) and maintained as above with media supplemented with 0.5 mg/mL G418
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