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Inflammatory gene expression, including TLR4, NFKB1, TNFA , neutrophil elastase (ELANE), H2A, CASP3 , and CASP7 , was largely unaffected by choline, though a greater reduction in TLR4 expression was observed at higher doses

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Even when employing a maleimide linker designed for enhanced stability, considerable thiazine formation has been noted, suggesting the ubiquitous nature of this side reaction