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After centrifugation, the collected cell membrane was solubilized with 20 mM Hepes, pH 7.5, 150 mM NaCl, 1% (w/v) sodium cholate, 0.05% dodecyl maltoside, 0.005% CHS (DDM/CHS Pre-Made Solutio, Anatrace), 5 mM -ME and 5 mM imidazole overnight at 4 C