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Closer examination revealed that the linker regions of both molecules adopt a distinctly bent conformation to facilitate covalent interaction with Cys13 (illustrated for eda-GDP (7OK3) in Fig

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The solution obtained was then centrifuged at 1000 g for 10 min at 4 C, and the supernatant was filtered through a 0.2-m nylon syringe filter for subsequent HPLC analysis 28