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Normal replication is seen, however, in cells infected with PV1-BSOr variants regardless of whether the cells were GSH-depleted or not (Figure 8A)

Cardiomyocyte fractional shortening, calcium transients Adult mouse left ventricular cardiomyocytes (LVCMs) were loaded with 0.5 M Fura2-AM and placed on the stage of an inverted microscope in a chamber containing normal physiological Tyrodes solution (150 mM NaCl, 5.4 mM KCl, 1.2 mM MgCl 2 , 10 mM glucose, 2 mM Na-pyruvate, 1.8 mM CaCl 2 , and 5 mM HEPES, pH 7.35) 100

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The most common subcutaneous injection sites include: Abdomen (Belly) Typically, the preferred site is at least two inches away from the navel