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After that, the cells were stained in 1 mM FerroOrange (Dojindo, Kumamoto, Japan) in HBSS for exactly 30 min at 37 C and 5% CO2 and imaged immediately with a fluorescence microscope

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McKone EF, Shao J, Frangolias DD, Keener CL, Shephard CA, Farin FM, Tonelli MR, Pare PD, Sandford AJ, Aitken ML, et al: Variants in the glutamate-cysteine-ligase gene are associated with cystic fibrosis lung disease

doi: 10.2903/j.efsa.2014.3844 89

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